Device

Part:BBa_K4408008:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)


Pthl-adhE2, Expression of adhE2 with Pthl promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our program, we use this device to construct the synthesis pathways of butanol in Clostridium tyrobutyricum. By introducing adhE2，an intermediate, butyryl-CoA, is converted to butyl aldehyde and further transformed to butanol. Pthl starts the transcription and Cpa fdx terminator ends the transcription.


Source

Pthl is based on part BBa_K3443002. RBS for adhE2 is based on BBa_K4408001. adhE2 is based on part BBa_K1462060. Cpa fdx terminator is based on part BBa_K3585002.

References